diff --git a/gdb/testsuite/gdb.base/cached-source-file.c b/gdb/testsuite/gdb.base/cached-source-file.c
new file mode 100644
index 0000000000..9f698dcffe
--- /dev/null
+++ b/gdb/testsuite/gdb.base/cached-source-file.c
@@ -0,0 +1,37 @@
+/* This testcase is part of GDB, the GNU debugger.
+
+   Copyright 2020 Free Software Foundation, Inc.
+
+   This program is free software; you can redistribute it and/or modify
+   it under the terms of the GNU General Public License as published by
+   the Free Software Foundation; either version 3 of the License, or
+   (at your option) any later version.
+
+   This program is distributed in the hope that it will be useful,
+   but WITHOUT ANY WARRANTY; without even the implied warranty of
+   MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+   GNU General Public License for more details.
+
+   You should have received a copy of the GNU General Public License
+   along with this program.  If not, see <http://www.gnu.org/licenses/>.  */
+
+/* Test for PR tui/25126.
+
+   The .exp testcase depends on the line numbers and contents from
+   this file  If you change this file, make sure to double-check the
+   testcase.  */
+
+#include <stdio.h>
+
+void
+foo (void)
+{
+  printf ("hello\n"); /* break-here */
+}
+
+int
+main ()
+{
+  foo ();
+  return 0;
+}
diff --git a/gdb/testsuite/gdb.base/cached-source-file.exp b/gdb/testsuite/gdb.base/cached-source-file.exp
new file mode 100644
index 0000000000..f98bec09ca
--- /dev/null
+++ b/gdb/testsuite/gdb.base/cached-source-file.exp
@@ -0,0 +1,94 @@
+# Copyright (C) 2020 Free Software Foundation, Inc.
+
+# This program is free software; you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation; either version 3 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program.  If not, see <http://www.gnu.org/licenses/>.
+
+# Test for PR tui/25126.
+# This bug is reproducible even without using the TUI.
+
+standard_testfile
+
+# Only run on native boards.
+if { [use_gdb_stub] || [target_info gdb_protocol] == "extended-remote" } {
+    return -1
+}
+
+# Because we need to modify the source file later, it's better if we
+# just copy if to our output directory (instead of messing with the
+# user's source directory).
+set newsrc [standard_output_file $testfile].c
+file copy -force -- $srcdir/$subdir/$srcfile $newsrc
+set srcfile $newsrc
+
+if { [prepare_for_testing "failed to prepare" $testfile $srcfile] } {
+    return -1
+}
+
+# Get the line number for the line with the "break-here" marker.
+set bp_line [gdb_get_line_number "break-here" $srcfile]
+
+if { [runto "$srcfile:$bp_line"] } {
+    pass "run to $srcfile:$bp_line"
+} else {
+    fail "run to $srcfile:$bp_line"
+}
+
+# Do a "list" and check that the printed line matches the line of the
+# original source file.
+gdb_test_no_output "set listsize 1"
+gdb_test "list" "$bp_line\[ \t\]+printf \\(\"hello\\\\n\"\\); /\\* break-here \\*/" \
+    "check the first version of the source file"
+
+# Modify the original source file, and add an extra line into it.
+# This only works locally because of the TCL commands.
+set bkpsrc [standard_output_file $testfile].c.bkp
+set bkpsrcfd [open $bkpsrc w]
+set srcfd [open $srcfile r]
+
+while { [gets $srcfd line] != -1 } {
+    if { [string first "break-here" $line] != -1 } {
+	# Put a "printf" line before the "break-here" line.
+	puts $bkpsrcfd "  printf (\"foo\\n\");"
+    }
+    puts $bkpsrcfd $line
+}
+
+close $bkpsrcfd
+close $srcfd
+file rename -force $bkpsrc $srcfile
+# Give it some time to perform the renaming.  For some reason, TCL
+# needs some time after "file rename" in order to properly rename the
+# file.
+sleep 1
+
+# Recompile the modified source.  We use "gdb_compile" here instead of
+# "prepare_for_testing" because we don't want to call "clean_restart".
+if { [gdb_compile "${srcfile}" "${binfile}" executable {debug}] != "" } {
+    return -1
+}
+
+# Rerun the program.  This should not only force GDB to reload the
+# source cache, but also to break at line 6 again, which now has
+# different contents.
+gdb_test_multiple "run" "rerun program" {
+    -re {The program being debugged has been started already\.\r\nStart it from the beginning\? \(y or n\) $} {
+	set binregex [string_to_regexp $binfile]
+	gdb_test "y" "\\`$binregex\\' has changed; re-reading symbols\.\r\nStarting program: ${binregex}.*" \
+	    "rerun program"
+    }
+}
+
+# Again, perform the listing and check that the line indeed has
+# changed for GDB.
+gdb_test "list" "${bp_line}\[ \t\]+printf \\(\"foo\\\\n\"\\);" \
+    "verify that the source code is properly reloaded"
